overview of our research



​Check out the University of Washington Laboratory Medicine Grand Rounds talk by Dr. Murphy describing our group's work. Click the video or follow this link.

SELECTED publications

Searchable at Pubmed (https://www.ncbi.nlm.nih.gov/pubmed)
​
​Molecular Diagnostics & Epidemiology
  • Murphy SC, Prentice JL, Williamson K, Wallis CK, Fang FC, Fried M, Pinzon C, Wang R, Talley AK, Kappe SHI, Duffy PE, Cookson BT. 2012. Real-time quantitative reverse transcription PCR for monitoring of blood-stage Plasmodium falciparum infections in malaria human challenge trials Am J Trop Med Hyg. 86(3):383–94. (https://doi.org/10.4269/ajtmh.2012.10-0658)
  • Murphy SC, Daza G, Chang M, Coombs RW. 2012. Laser cutting eliminates nucleic acid cross-contamination in dried blood spot processing. J Clin Microbiol. 50(12):4128-30. (https://doi.org/10.1128/JCM.02549-12)
  • Murphy SC, Shott JP, Parikh S, Etter P, Prescott WR, Stewart VA. 2013. Malaria Diagnostics in Clinical Trials. Am J Trop Med Hyg. 89(5):824-839. (https://doi.org/10.4269/ajtmh.12-0675)
  • Murphy SC, Hermsen CC, Douglas AD, Edwards NJ, Petersen P, Fahle GA, Adams M, Berry AA, Billman ZP, Gilbert SC, Laurens MC, Leroy O, Lyke KE, Plowe CV, Seilie AM, Strauss KA, Teelen K, Hill AVS, Sauerwein RW. 2014. External quality assurance of malaria nucleic acid testing for clinical trials and eradication surveillance PLoS ONE 9(5): e97398. (https://doi.org/10.1371/journal.pone.0097398)
  • Hodgson SH, Douglas AD, Edwards NJ, Kimani D, Elias SC, Chang M, Daza G, Seilie AM, Magiri C, Muia A, Juma EA, Cole AO, Rampling TW, Anagnostou NA, Gilbert SC, Hoffman SL, Draper SJ, Bejon P, Ogutu B, Marsh K, Hill AVS, Murphy SC. 2015. Increased sample volume and use of quantitative reverse-transcription PCR can improve prediction of liver-to-blood inoculum size in controlled human malaria infection studies. Malaria J 14:33. (https://doi.org/10.1186/s12936-015-0541-6) (PMC4318195)
  • Fang FC, Billman ZP, Wallis C, Abbott A, Olson J, Dhanireddy S, Murphy SC. 2015. Human Diphyllobothrium nihonkaiense infestation in Washington State. J Clin Microbiol 53(4):1355-7. (https://doi.org/10.1128/JCM.00065-15) (PMCID: PMC4365193)
  • Hanron AH, Billman ZP, Seilie AM, Chang M, Murphy SC. 2016. Detection of Babesia microti parasites by highly sensitive 18S rRNA reverse transcription PCR. Diag Microbiol Infect Dis. 87(3):226-8. (https://doi.org/10.1016/j.diagmicrobio.2016.11.021)
  • Hanron AE, Billman ZP, Seilie AM, Olsen TM, Fishbaugher M, Chang M, Rueckle T, Andenmatten N, Greenhouse B, Arinaitwe E, Rek J, Das S, Domingo GJ, Shipman K, Kappe SH, Kublin JG, Murphy SC. 2017. Multiplex, DNase-free one-step reverse transcription PCR for Plasmodium 18S rRNA and spliced gametocyte-specific mRNAs. Malaria J 16(1):208. (https://doi.org/10.1186/s12936-017-1863-3) (PMC5438523).
  • Das S, Jang IK, Barney B, Peck R, Rek J, Arinaitwe E, Adama H, Murphy M, Imwong M, Ling C, Proux S, Haohankhunnatham W, Rist M, Seilie AM, Hanron A, Daza G, Chang M, Nakamura T, Kalnoky M, Labarre P, Murphy SC, McCarthy J, Nosten F, Greenhouse B, Domingo GJ. 2017. Performance of a high-sensitivity rapid diagnostic test for Plasmodium falciparum malaria in asymptomatic individuals from Uganda and Myanmar and naïve human challenge infections. Am J Trop Med Hyg 97(5):1540-1550 (PMID 28820709) (https://doi.org/10.4269/ajtmh.17-0245)
  • Fahle GA, Luethy PM, Murphy SC, Seilie AM, Xie YL, Lau C, Tisdale JF, Hsieh MM, Reinhardt JL, Lau AF. 2018. Case Report: Diagnostic challenges of prolonged post-treatment clearance of Plasmodium nucleic acids in a pre-transplant autosplenectomized patient with sickle cell disease. Malaria J. 17:23 (https://doi.org/10.1186/s12936-017-2152-x)
  • Murphy SC, Ishizuka AS, Billman ZP, Olsen TM, Seilie AM, Chang M, Smith N, Chuenchob V, Chakravarty S, Sim BKL, Kappe SHI, Hoffman SL, Seder RA. 2018. Plasmodium 18S rRNA of intravenously administered sporozoites does not persist in peripheral blood. Malaria J 17:275. (https://doi.org/10.1186/s12936-018-2422-2)
  • Plucinski MM, Herman C, Jones S, Dimbu R, Fortes F, Ljolje D, Lucchi N, Murphy SC, Smith NT, Cruz KR, Seilie AM, Halsey ES, Udhayakumar V, Aidoo M, Rogier E. 2019. Screening for Pfhrp2/3-Deleted Plasmodium falciparum, Non-falciparum, and Low-Density Malaria Infections by a Multiplex Antigen Assay. J Infect Dis. 219:437-47. (https://doi.org/10.1093/infdis/jiy525)
  • Plucinski MM, Dimbu R, Fortes F, Murphy SC, Smith NT, Cruz KR, Seilie AM, Halsey ES, Aidoo M, Rogier E. 2019. Malaria parasite density in individuals with different rapid diagnostic test results and concentrations of HRP2 antigen. Amer J Trop Med Hyg 100(5):1202-3. (https://doi.org/10.4269/ajtmh.19-0006). 
  • Seilie AM, Chang M, Hanron AE, Billman ZP, Stone BC, Zhou K, Olsen TM, Daza G, Ortega J, Cruz K, Smith NT, Healy SA, Neal J, Wallis CK, Fritzen E, Shelton L, VonGoedert T, Wong-Madden S, Kappe SHI, Fishbaugher M, Betz W, Hume JCC, Talley AK, Wald A, Plowe CV, Adams M, Fahle GA, Cowan E, Duffy PE, Kublin JG, Murphy SC. 2019. Beyond blood smears for malaria - clinical validation of the Plasmodium 18S rRNA as a biomarker for controlled human malaria infections. Amer J Trop Med Hyg 100(6):1466-1476. (https://doi.org/10.4269/ajtmh.19-0094)
    • Report on the successful FDA Biomarker Qualification of the Plasmodium 18S rRNA for a non-endemic controlled human malaria infection context of use. First malaria biomarker qualified at the FDA in this manner.
  • Jang IK, Tyler A, Lyman C, Kahn M, Kalnoky M, Rek J, Arinaitwe E, Adrama H, Murphy M, Imwong M, Ling C, Proux S, Haohankhunnatham W, Rist M, Seilie A, Hanron A, Daza G, Chang M, Das S, Barney R, Rashid A, Landier J, Boyle D, Murphy S, McCarthy J, Nosten F, Greenhouse B, Domingo G. 2018. Simultaneous Quantification of Plasmodium Antigens and Host Factor CRP in Asymptomatic Individuals with Confirmed Malaria Using a Novel Multiplex Immunoassay. J Clin Microbiol JCM.00948-18 (https://doi.org/10.1128/JCM.00948-18)
  • Cunningham JA, Thomson RM, Murphy SC, de la Paz Ade M, Ding XC, Incardona S, Legrand E, Lucchi NW, Menard D, Nsobya SL, Saez AC, Chiodini PL, Shrivastava J. 2020. WHO malaria nucleic acid amplification test external quality assessment scheme: results of distribution programmes one to three. Malaria J. 19(1):129. (https://doi.org/10.1186/s12936-020-03200-0). PMID: 32228615.​
  • Jimmy OT, Emmanuel O, Gonzaga A, Basil O, Jimmy L, Murphy SC, Egwang TG. 2020. Using the ultrasensitive Alere Pf Malaria Ag HRP-2TM rapid diagnostic test in the field and clinic in northeastern Uganda. Amer J Trop Med Hyg. 103(2):778-784. https://doi.org/10.4269/ajtmh.19-0653. PMID: 32602431
  • Vasquez AM, Velez GJ, Medina AC, Serra-Casas E, Campillo A, Gonzalez IJ, Murphy SC, Seilie AM, Tobon-Castano A. Evaluation of highly sensitive diagnostic tools for the detection of P. falciparum in pregnant women attending antenatal care visits in Colombia. BMC Preg Childbirth. 20(1):440. https://doi.org/10.1186/s12884-020-03114-4. PMID: 32736543
  • Francis A, Shears M, Murphy SC, Fu D. Direct quantification of single red blood cell hemoglobin concentration with multiphoton microscopy. Analytical Chem. 92(18):12235-12241. https://doi.org/10.1021/acs.analchem.0c01609. PMID: 32786430.
  • Glasgow HK, Cruz K, Murphy SC. 2021. Reverse-transcription PCR increases sensitivity of broad-range fungal detection in bronchoalveolar lavage fluid. Med Mycol. myab061, https://doi.org/10.1093/mmy/myab061
  • Wang CYT, Ballard E, Pava Z, Marquart L, Gaydon J, Murphy SC, Whiley D, O’Rourke P, McCarthy JS. 2021. Analytical validation of a real-time hydrolysis probe PCR assay for quantifying Plasmodium falciparum parasites in experimentally infected human adults. Malar J 20:181 https://doi.org/10.1186/s12936-021-03717-y. PMID: 33838672
  • Hergott DEB, Balkus JE, García GA, Cruz KR, Seilie AM, Masters H, Aveika AA, Iyanga MM; Eyono JNM, Guthrie BL, Murphy SC. 2021. Clustering of subpatent infections in households with asymptomatic rapid diagnostic test-positive cases in Bioko Island, Equatorial Guinea independent of travel to regions of higher malaria endemicity: a cross-sectional study. Malaria J. 20(1):313. https://doi.org/10.1186/s12936-021-03844-6. PMID: 34247643
  • Chang M, Johnson S, Seilie AM, Hergott D, Murphy SC. 2021. Application of dried blood spot sample pooling strategies for Plasmodium 18S rRNA biomarker testing to facilitate identification of infected persons in large-scale epidemiological studies. Malar J https://doi.org/10.1186/s12936-021-03907-8. PMID: 34620192
  • Hergott DEB, Owalla TJ, Balkus JE, Apio B, Lema J, Cemeri B, Akileng A, Seilie AM, Chavtur C, Staubus W, Chang M, Egwang TG, Murphy SC. 2022. Feasibility of community at-home dried blood spot collection combined with pooled reverse transcription PCR as a viable and convenient method for malaria epidemiology studies. Malar J 221. (https://doi.org/10.1186/s12936-022-04239-x). PMID: 35836179
  • Matsubara J, Chang M, Seilie AM, Murphy SC. 2022. Flow cytometric sorting of infected erythrocytes demonstrates reliable detection of individual ring-stage Plasmodium falciparum parasites by Plasmodium 18S rRNA reverse transcription PCR. Am J Trop Med Hyg. 11:tpmd211226. (https://doi.org/10.4269/ajtmh.21-1226). PMID: 35405648.
  • Gualdrón-López M, Díaz-Varela M, Zanghi G, Aparici-Herraiz I, Steel RJW, Schäfer C, Cuscó P, Vorada Chuenchob V, Kangwangransan N, Billman ZP, Olsen TM, González JR, Roobsoong W, Sattabongkot J, Murphy SC, Mikolajczak SA, Fernandez-Becerra C, Flannery EL, Kappe SHI, del Portillo HA. 2022. Mass spectrometry identification of biomarkers in extracellular vesicles from Plasmodium vivax hypnozoite infections. Mol Cel Proteom. 21(10):100406. https://doi.org/10.1016/j.mcpro.2022.100406. PMID 36030044.
  • Chavtur C*, Staubus WJ*, Ho M, Hergott DEB, Seilie AM, Healy S, Duffy P, Jackson L, Talley A, Kappe SHI, Hoffman SL, Richie TL, Kublin JG, Chang M, Murphy SC. 2023. Plasmodium 18S rRNA biomarker clearance after FDA-approved antimalarial treatment in controlled human malaria infection trials. Open Forum Infect Dis. 10(5):ofad202. https://doi.org/10.1093/ofid/ofad202. PMID: 37265668. (*co-first authors)

Pre-clinical Malaria Drug/Vaccine Development
  • Murphy SC, Kas A, Stone BC, Bevan MJ. 2013. A cytotoxic T cell response to a liver-stage Plasmodium antigen is not boosted by repeated sporozoite immunizations. Proc Natl Acad Sci USA 110(15): 6055–6060. (https://doi.org/10.1073/pnas.1303834110)
  • Douglass AN, Kain HS, Abdullahi M, Arang N, Austin LS, Mikolajczak SA, Billman ZP, Hume JC, Murphy SC, Kappe SH, Kaushansky A. 2015. Host-based prophylaxis successfully targets liver stage malaria parasites. Mol Ther. 23(5):857-65. (https://doi.org/10.1038/mt.2015.18) (PMC4427874)
  • Stone BC, Kas A, Billman ZP, Murphy SC. 2016. Complex synthetic minigene T cell vaccination for Plasmodium antigen discovery. PLoS One. 11(4):e0153449. (https://doi.org/10.1371/journal.pone.0153449). PMC4829254
  • Billman, ZP, Kas A, Stone BC, Murphy SC. 2016. Defining rules of CD8+ T cell expansion against pre-erythrocytic Plasmodium antigens in sporozoite-immunized mice. Malaria J 15(1):238. (https://doi.org/10.1186/s12936-016-1295-5).
  • Billman ZP, Seilie AM, Murphy SC. 2016. Purification of Plasmodium sporozoites enhances parasite-specific T cell responses Infect Immun 84(8):2233-42. (https://doi.org/10.1128/IAI.01439-15).
  • Sack BK, Mikolajczak SA, Fishbaugher M, Vaughan AM, Flannery EL, Nyugen T, Betz W, Navarro MJ, Foquet L, Steel RWJ, Billman ZP, Murphy SC, Hoffman SL, Chakravarty S, Sim BKL, Behet M, Reuling IJ, Walk J, Scholzen A, Sauerwein RW, Ishizuka AS, Flynn B, Seder RA, Kappe SHI. 2017. Humoral protection against mosquito bite-transmitted Plasmodium falciparum infection in humanized mice. NPJ Vaccines 2 (article 27. (https://doi.org/10.1038/s41541-017-0028-2).
  • Flannery EL, Foquet L, Chuenchob V, Fishbaugher M, Billman ZP, Foquet L, Navarro MJ, Betz W, Olsen TO, Lee J, Scafer C, Sack B, Wilson E, Bial J, Campo B, Charman S, Saunders J, Murphy SC, Phillips MA, Kappe SHI, Mikolajczak S. 2018. Assessment of drug efficacy against P. falciparum liver stages in a liver-chimeric humanized mouse model. JCI Insight 3(1). pii: 92587 (https://doi.org/10.1172/jci.insight.92587)
  • Kisalu N, Idris A, Weidle C, Flores-Garcia Y, Flynn B, Sack B, Murphy SC, Kappe S, Schön A, Freire E, Francica J, Miller A, Riera S, Kanekiyo M, Wheatley A, McDermott A, Farney SK, Chuang G, Zhang B, Natasha KC, Chakravarty S, Kwong P, Bhatia S, Sim BKL, Hoffman S, Zavala F, Pancera M, Seder R. 2018. A human monoclonal antibody prevents malaria infection and defines a site of vulnerability on Plasmodium falciparum circumsporozoite protein. Nature Med 24(4):408-416. (https://doi.org/10.1038/nm.4512)
  • Foquet L, Schafer C, Minkah NK, Alanine DGW, Flannery EL, Steel RWJ, Camargo N, Fishbaugher M, Betz W, Nguyen T, Billman ZP, Wilson EM, Bial J, Murphy SC, Draper SJ, Mikolajczak SA, Kappe SHI. 2018. Plasmodium falciparum liver stage infection and transition to stable blood stage infection in liver-humanized and blood-humanized FRGN KO mice. Frontiers Immunol 9:524 (https://doi.org/10.3389/fimmu.2018.00524)
  • Olsen TM, Stone BC, Chuenchob V, Murphy SC. 2018. Prime-and-trap malaria vaccination to generate protective CD8+ liver resident memory T cells. J Immunology. 201(7) 1984-1993. (https://doi.org/10.4049/jimmunol.1800740
  • Shears M, Murphy SC. Vancomycin improves Plasmodium yoelii malaria parasite in vitro liver stage cultures by controlling Elizabethkingia anophelis, a bacterium in the microbiome of lab-reared Anopheles mosquitoes. Mol Biochem Parasitol 111279 (https://doi.org/10.1016/j.molbiopara.2020.111279). PMID: 32360511​ 
  • Goswami D, Betz W, Kamal N, Parthiban C, Brager C, Schäfer C, Camargo N, Nguyen T, Murphy SC, Vaughan A, Kappe S. 2020. A replication-competent late liver stage-attenuated human malaria parasite. JCI Insight https://doi.org/10.1172/jci.insight.135589
  • Schäfer S, Roobsoong W, Kangwanrangsan N, Bardelli M, Rawlinson TA, Dambrauskas N, Trakhimets O, Parthiban C, Goswami D, Reynolds LM, Kennedy SY, Flannery EL, Murphy SC, Sather DN, Draper SJ, Sattabongkot J, Mikolajczak SA, Kappe SHI. 2020. A humanized mouse model for Plasmodium vivax to test interventions that block liver stage to blood stage transition and blood stage infection. iScience. 23(8):101381. https://doi.org/10.1016/j.isci.2020.101381. PMID: 32739836
  • Shears MJ, Seilie AM, Sim BKL, Hoffman SL, Murphy SC. Quantification of Plasmodium knowlesi versus Plasmodium falciparum in the rhesus liver: implications for malaria vaccine studies in rhesus models. 19(1):313. https://doi.org/10.1186/s12936-020-03385-4. PMID: 32867784.
  • Watson FN, Shears MJ, Matsubara J, Kalata AC, Seilie AM, Talavera IC, Olsen TM, Tsuji M, Chakravarty S, Sim BKL, Hoffman SL, Murphy SC. 2022. Cryopreserved sporozoites with and without the glycolipid adjuvant 7DW8-5 protect in prime-and-trap malaria vaccination. Am J Trop Med Hyg. PMID: 35226868 https://doi.org/10.4269/ajtmh.21-1084.
  • Goswami D, Kumar S, Betz W, Armstrong JA, Haile MT, Camargo N, Parthiban C. Seilie AM, Murphy SC, Vaughan AM, Kappe S. 2022. A Plasmodium falciparum ATP binding cassette transporter is essential for liver stage entry into schizogony. iScience 8;25(5):104224. (https://doi.org/10.1016/j.isci.2022.104224): PMID: 35521513
  • Wilder BK, Vigdorovich V, Carbonetti S, Minkah N, Hertoghs N, Raappana A, Cardamone H, Oliver BG, Trakhimets O, Kumar S, Dambrauskas N, Arredondo SA, Camargo N, Murphy SC, Kappe SHI, Sather DN. 2022. Anti-TRAP/SSP2 monoclonal antibodies can inhibit sporozoite infection and enhance protection of anti-CSP monoclonal antibodies. NPJ Vaccines. 7(1):58. (https://doi.org/10.1038/s41541-022-00480-2). PMID: 35618791 
  • Kimingi HW, Kinyua AW, Achieng NA, Wambui KM, Mwangi S, Nguti R, Kivisi CA, Jensen ATR, Bejon P, Kapulu MC, Abdi AI, Kinyanjui SM; CHMI-SIKA Study Team (including Murphy SC). 2022. Breadth of antibodies to Plasmodium falciparum variant surface antigens is associated with immunity in a controlled human malaria infection study. Front Immunol. 13:894770. (https://doi.org/10.3389/fimmu.2022.894770). PMID: 35711446.
  • Zhu Y, Shen R, Vuong I, Reynolds RA, Shears MJ, Yao Z-C, Hu Y, Cho WJ, Kong J, Reddy SK, Murphy SC, Mao H-Q. 2022. Multi-step screening and composition optimization of lipid nanoparticles for prolonged liver-targeted DNA delivery. Nat Comm 13:4282. (https://doi.org/10.1038/s41467-022-31993-y). 
  • Chakravarty S, Shears MJ, James ER, Rai U, KC N, Murphy SC, Hoffman SL. 2022. Efficient infection of non-human primates with purified, cryopreserved Plasmodium knowlesi sporozoites. Malaria J. 21(1):247. (https://doi.org/10.1186/s12936-022-04261-z). PMID: 36030292.
  • Flannery EL, Kangwangrangsan N, Chuenchob V, Roobsong W, Fishbugher M, Zhou K, Billman ZP, Martinson T, Olsen TM, Schafer C, Campo B, Murphy SC, Mikolajczak SA, Kappe SHI. Sattabongkot J. 2022. Plasmodium vivax latent liver infection is characterized by persistent hypnozoites, hypnozoite-derived schizonts, and time-dependent efficacy of primaquine. Molec Ther 26:427-40. (https://doi.org/10.1016/j.omtm.2022.07.016). PMID: 36092359.
  • Saveria T*, Parthiban C*, Seilie AM, Roberts J, Stone BC, Tasch M, Murphy SC. 2022. A needle-free, mucosally-delivered spirulina platform Plasmodium falciparum circumsporozoite vaccine provides sterile protection against pre-erythrocytic malaria in mice. npj Vaccines. 7:113. (https://doi.org/10.1038/s41541-022-00534-5) (*co-first authors). PMID 36195607.
  • Franke-Fayard B, Marin-Mogollon C, Geurten F, Chevalley-Maurel S, Ramesar J, Kroeze H, Baalbergen EE, Wessels E, Soulard V, Martinson T, Aleshnick M, Huijs A, Subudhi A, Miyazaki Y, Othman A, Kolli S, Lamers O, Roques M, Stanway R, Murphy S, Foquet L, Mendes A, Prudencio M, Dechering K, Heussler V, Pain A, Wilder B, Roestenberg M, Janse C. 2022. Generation and preclinical evaluation of a genetically attenuated malaria parasite, GA2, that arrests growth late in the liver. npj Vaccines. 7(1):139. https://doi.org/10.1038/s41541-022-00558-x. PMID: 36333336.
  • Watson FN, Duncombe CJ, Kalata AC, Conrad E, Chakravarty S, Hoffman SL, Tsuji M, Shears MJ, Murphy SC. 2023. Sex-specific differences in cytokine induction by the glycolipid adjuvant 7DW8-5 in mice. Biomolecules 13, 8. https://doi.org/10.3390/biom13010008. PMID: 36671393.
  • Shears MJ, Watson FN, Stone B, Cruz Talavera I, Parthiban C, Matsubara J, KC N, Sim BKL, Hoffman SL, Murphy SC. 2023. Preliminary studies on the immunogenicity of a prime-and-trap malaria vaccine in nonhuman primates. Vaccine. 41(38):5494-5498. https://doi.org/10.1016/j.vaccine.2023.07.067. PMID: 37563050.
  • Zhu Y, Shen R, Ma J, Vuong I, Kong J, Yao Z-C, Lin J, Wei C, Reynolds RA, Shears MJ, Cho WJ, Livingston NK, Li S, Howard GP, Hu Y, Choy J, Tzeng SY, Green JJ, Schneck JP, Reddy SK, Murphy SC, Mao H-Q. 2023. Screening for lipid nanoparticles that modulate the immune activity of helper T cells towards enhanced antitumour activity. Nat Biomed Eng. https://doi.org/10.1038/s41551-023-01131-0.
  • Yadav N, Parthiban C, Billman ZP, Stone B, Watson FN, Zhou K, Olsen TM, Cruz Talavera I, Seilie AM, Kalata AC, Matsubara J, Shears MJ, Reynolds R, Murphy SC. 2023. More time to kill: a longer liver stage increases T cell-mediated protection against pre-erythrocytic malaria. iScience 26(12):108489. PMID: 38162031. https://www.cell.com/iscience/fulltext/S2589-0042(23)02566-X.
  • Shears MJ, Reynolds RA, Duncombe CJ, Watson FN, Staubus WJ, Chavtur C, Seilie AM, Tran TM, Chakravarty S, Hoffman SL, Murphy SC. 2023. Plasmodium knowlesi in pig-tailed macaques: a potential new model for malaria vaccine research. Malaria J 22:379. PMID: 38093306. https://doi.org/10.1186/s12936-023-04788-9.
  • MacMillen Z, Hatzakis K, Simpson A, Shears MJ, Watson F, Erasmus JH, Khandhar AP, Wilder B, Murphy SC, Reed SG, Davie JW, Avril M. 2024. Accelerated prime-and-trap vaccine regimen in mice using repRNA-based CSP malaria vaccine. npj Vaccines 9, 12. PMID: 38200025. https://doi.org/10.1038/s41541-023-00799-4.
  • Watson FN, Shears MJ, Kalata AC, Duncombe CJ, Seilie AM, Chavtur C, Conrad E, Cruz Talavera I, Raappana A, Sather DN, Chakravarty S, Sim BKL, Hoffman SL, Tsuji M, Murphy SC. 2024. Ultra-low volume intradermal administration of radiation-attenuated sporozoites with the glycolipid adjuvant 7DW8-5 completely protects mice against malaria. Sci Rep. 14(1):2881. PMID: 38311678. https://doi.org/10.1038/s41598-024-53118-9.
  • Goswami D, Patel H, Betz W, Armstrong J, Camargo N, Patil A, Murphy SC, Sim BKL, Vaughan AM, Hoffman SL, Kappe SHI. 2024. A replication competent Plasmodium falciparum parasite strain completely attenuated by dual gene deletion. EMBO Molec Med. 16(4):723-54. PMID: 38514791.
  • Goswami D, Arredondo SA, Betz W, Armstrong J, Kumar S, Patel H, Camargo N, Oualim KMZ, Seilie AM, Murphy SC, Kappe SHI, Vaughan AM. 2024. A conserved Plasmodium nuclear protein is critical for late liver stage development. Commun Biol 7(1):1387. https://doi.org/10.1038/s42003-024-07063-y. PMID: 39455824.
  • Yadav N, Kalata AC, Reynolds RA, Raappana A, Sather DN, Murphy SC. Identifying Plasmodium P36 and P52 antigens for co-administration with circumsporozoite protein to enhance vaccine efficacy. npj Vaccines. 9, 241 (2024). https://doi.org/10.1038/s41541-024-01040-6. PMID: 39643623

Malaria Clinical Trials
  • Talley AK, Healy SA, Finney OC, Murphy SC, Kublin J, Salas CJ,, Lundebjerg S, Gilbert P, Whisler J, Van Voorhis WC, Wang R, Murphy JR, Ockenhouse CF, Heppner DG, Kappe SH, Duffy PE. 2014. Safety and comparability of controlled human Plasmodium falciparum infection by mosquito bite in malaria-naïve subjects at a new facility for sporozoite challenge. PLoS ONE 9(11): e109654. (https://doi.org/10.1371/journal.pone.0109654)
  • Kublin JG, Mikolajczak SA, Sack BK, Fishbauger M, Seilie A, Shelton L, VonGoederdt T, Firat M, Magee S, Fritzen E, Betz W, Kain HS, Dankwa DA, Steel, RWJ, Vaughan AM, Sather DN, Murphy SC, Kappe SHI. 2017. Complete attenuation of a genetically-engineered Plasmodium falciparum parasite in humans. Sci Transl Med 9(371): (https://doi.org/10.1126/scitranslmed.aad9099)
  • Murphy SC, Duke ER, Shipman KJ, Jensen RL, Fong Y, Ferguson S, Janes HE, Gillespie K, Seilie AM, Hanron AE, Rinn L, Fishbaugher M, VonGoedert T, Fritzen E, Kappe SH, Chang M, Sousa JC, Marcsisin SR, Chalon S, Duparc S, Kerr N, Möhrle JJ, Andenmatten N, Rueckle T, Kublin JG. 2018. A randomized trial of the prophylactic activity of DSM265 against pre-erythrocytic Plasmodium falciparum controlled human malaria infection by mosquito bites and direct venous inoculation. J Infect Dis 217(5):693-702. (https://doi.org/10.1093/infdis/jix613).
  • Healy SA, Murphy SC, Hume JCC, Shelton L, Kuntz S, Van Voorhis WC, Moodie Z, Metch B, Wang R, Silver-Brace T, Fishbaugher M, Kennedy M, Finney OC, Chaturvedi R, Hobbs CV, Warner-Lubin M, Talley AK, Wong-Madden S, Stuart K, Wald A, Kappe SH, Kublin JG, Duffy PE. 2019. Chemoprophylaxis vaccination: Phase 1 study to explore stage-specific immunity to Plasmodium falciparum in U.S. adults. Clin Infect Dis ciz1010. (http://doi.org/10.1093/cid/ciz1010). PMID: 31621832.
  • Wockner LF, Hoffmann I, Webb L, Mordmüller B, Murphy SC, Kublin JG, O'Rourke P, McCarthy JS, Marquart L. 2020. Growth Rate of Plasmodium falciparum: Analysis of Parasite Growth Data From Malaria Volunteer Infection Studies. J Infect Dis 221(6):963-972. (https://doi.org/10.1093/infdis/jiz557). PMID: 31679015. 
  • Kublin J, Murphy SC, Maenza J, Seilie AM, Jain JP, Goswami B, Berger D, Spera D, Stein DS, Zhao R, Hackling M, Soon RL, Czartoski JL, Potochnic MA, Duke E, Chang M, Vaughan A, Kappe SHI, Leong FJ, Pertel P. Prince WT. 2020. Safety, pharmacokinetics and causal prophylactic efficacy of KAF156 in a Plasmodium falciparum human infection study. Clin Infect Dis. ciaa952 (https://doi.org/10.1093/cid/ciaa952).
  • Murphy SC, Deye GA, Sim BKL, Galbiati S, Kennedy JK, Cohen KW, Chakravarty S, KC N, Abebe Y, James ER, Kublin JG, Hoffman SL, Richie TL, Jackson LA. 2021. PfSPZ-CVac efficacy against malaria increases from 0% to 75% when administered in the absence of erythrocyte stage parasitemia: a randomized, placebo-controlled trial with controlled human malaria infection. PLoS Pathog. 17(5): e1009594. PMID: 34048504.
  • Mwakingwe-Omari A, Healy SA, Lane J, Cook DM, Kalhori S, Wyatt C, Kolluri A, Marte-Salcedo O, Imeru A, Nason M, Ding LK, Decederfelt H, Duan J, Neal J, Raiten J, Lee G, Hume JCC< Jeon JE, Ikpeama I, KC N, Chakravarty S, Murshedkar T, Church LWP, Manoj A, Gunasekera A, Anderson C, Murphy SC, March S, Bhatia SN, James ER, Billingsley PF, Sim BKL, Richie TL, Zaidi I, Hoffman SL, Duffy PE. 2021. Two chemoattenuated PfSPZ malaria vaccines induce sterile hepatic immunity. Nature. 595(7866):289-294. https://doi.org/10.1038/s41586-021-03684-z. PMID: 34194041.
  • Pal S, Myburgh J, Bansil P, Hann A, Robertson L, Gerth-Guyette E, Ambler G, Bizilj G, Kahn M, Zobrist S, Manis MR, Styke NA, Allan V, Ansbro R, Akingbade T, Bryan A, Murphy SC, Kublin JG, Layton M, Domingo GJ. 2021. Reference and point-of-care testing for G6PD deficiency: Blood disorder interference, contrived specimens, and fingerstick equivalence and precision. PLoS One 16(9):e0257560. https://doi.org/10.1371/journal.pone.0257560. PMID: 34543346.
  • Shaffer D, Kumwenda J, Chen H, Akelo V, Angira F, Kosgei J, Tonui R, Ssali F, McKhann A, Hogg E, Stewart VA, Murphy SC, Coombs R, Schooley R; A5297 Team. 2022. Brief Report: No Differences Between Lopinavir/ritonavir and non-Nucleoside Reverse Transcriptase Inhibitor-based Antiretroviral Therapy on Clearance of Plasmodium falciparum Subclinical Parasitemia in Adults Living With HIV Starting Treatment (A5297). J Acquir Immune Defic Syndr. 89(2):178-182. https://doi.org/10.1097/QAI.0000000000002839. PMID: 34693933.
  • Chi P, Awuor Owino E, Jao I, Olewe F, Ogutu B, Bejon P, Kapulu M, Kamuya D, Marsh V, CHMI-SIKA Study Team (including Murphy SC). 2021. Understanding the benefits and burdens associated with a malaria human infection study in Kenya: experiences of study volunteers and other stakeholders. 2021. Trials. 22(1):494. doi: 10.1186/s13063-021-05455-7. (https://doi.org/10.1186/s13063-021-05455-7). PMID: 34311781.
  • Kapulu MC, Njuguna P, Hamaluba M, Kimani D, Ngoi JM, Musembi J, Ngoto O, Otieno E, Billingsley PF, Controlled Human Malaria Infection in Semi-Immune Kenyan Adults (CHMI-SIKA) Study Team (including Murphy SC). 2021. Safety and PCR monitoring in 161 semi-immune Kenyan adults following controlled human malaria infection. JCI Insight. 6(17):146443. (https://doi.org/10.1172/jci.insight.146443). PMID: 34264864. 
  • Kimingi HW, Kinyua AW, Achieng NA, Wambui KM, Mwangi S, Nguti R, Kivisi CA, Jensen ATR, Bejon P, Kapulu MC, Abdi AI, Kinyanjui SM; CHMI-SIKA Study Team (including Murphy SC). 2022. Breadth of antibodies to Plasmodium falciparum variant surface antigens is Associated With Immunity in a Controlled Human Malaria Infection Study. Front Immunol. 13:894770. https://doi.org/10.3389/fimmu.2022.894770. PMID: 35711446; PMCID: PMC9195513.
  • Kariuki SN, Macharia AW, Makale J, Nyamu W, Hoffman SL, Kapulu MC, Bejon P, Rayner JC, Williams TN; for the CHMI-SIKA Study Team (including Murphy SC). 2023. The Dantu blood group prevents parasite growth in vivo: Evidence from a controlled human malaria infection study. Elife. 12:e83874. https://doi.org/10.7554/eLife.83874. PMID: 37310872; PMCID: PMC10264070.
  • Murphy SC, Vaughan AM, Kublin JG, Fishbauger M, Seilie AM, Cruz KP, Mankowski T, Firat M, Magee S, Betz W, Kain H, Camargo N, Haile MT, Gibson J, Fritzen E, Hertoghs N, Sather DN, Deye GA, Galbiati S, Geber C, Butts J, Jackson LbiA, Kappe SHI 2022. A genetically-engineered Plasmodium falciparum parasite vaccine provides protection from controlled human malaria infection. Sci Transl Med. 14(659):eabn9709. (https://doi.org/10.1126/scitranslmed.abn9709). PMID: 36001680.
  • Owalla TJ, Hergott D, Seilie AM, Staubus W, Chavtur C, Chang M, Kublin JG, Egwang TG, Murphy SC. 2022. Rethinking detection of pre-existing and intervening Plasmodium infections in malaria clinical trials. Front Immunol. (https://doi.org/10.3389/fimmu.2022.1003452). PMID 36203582.   
  • Hergott DEB, Owalla TJ, Seilie AM, Chavtur C, Staubus W, Balkus JE, Apio B, Lema J, Cemeri B, Akileng A, Chang M, Egwang TG, Murphy SC. Assessing the daily natural history of asymptomatic Plasmodium infections in adults and older children in Katakwi, Uganda: a longitudinal cohort study. 2024. Lancet Microbe. Epub 04 Jan 2024. PMID: 38185134. https://doi.org/10.1016/S2666-5247(23)00262-8.
  • Golden A, Slater HC, Jang IK, Walke S, Phan TT, Bizilj GT, Rashid A, Barney R, Das S, Rist MJ, McCarthy JS, Nosten F, Landier J, Imwong M, Hume JCC, Sagara I, Healy SA, Duffy PE, Ntuku H, Mumbengegwi D, Hsiang MS, Murphy SC, Rek J, Torres K, Gamboa D, Domingo GJ. Analytical Sensitivity Analysis and Clinical Impact Modeling of Rapigen Rapid Diagnostic Tests for Malaria. Am J Trop Med Hyg. 2024 Sep 3:tpmd240003. https://doi.org/10.4269/ajtmh.24-0003. PMID: 39226907
  • Hergott DEB, Guerra CA, García GA, Mba Eyono JN, Donfack OT, Iyanga MM, Nguema Avue RM, Abeso Nsegue CN, Ondo Mifumu TA, Rivas MR, Phiri WP, Murphy SC, Guthrie BL, Smith DL, Balkus JE. 2024. Impact of six-month COVID-19 travel moratorium on Plasmodium falciparum prevalence on Bioko Island, Equatorial Guinea. Nat Commun. 15(1):8285. https://doi.org/10.1038/s41467-024-52638-2. PMID: 39333562
  • Odera DO, Tuju J, Mwai K, Nkumama IN, Fürle K, Chege T, Kimathi R, Diehl S, Musasia FK, Rosenkranz M, Njuguna P, Hamaluba M, Kapulu MC, Frank R; CHMI-SIKA Study Team; Osier FHA, Abdi AI, Chi PC, de Laurent Z, Jao I, Kamuya D, Kamuyu G, Makale J, Murungi L, Musyoki J, Muthui M, Mwacharo J, Kariuki S, Mwanga D, Mwongeli J, Ndungu F, Njue M, Nyangweso G, Kimani D, Ngoi JM, Musembi J, Ngoto O, Otieno E, Ooko M, Shangala J, Wambua J, Mohammed KS, Omuoyo D, Mosobo M, Kibinge N, Kinyanjui S, Bejon P, Lowe B, Marsh K, Marsh V, Abebe Y, Billingsley PF, Sim BKL, Hoffman SL, James ER, Richie TL, Audi A, Olewe F, Oloo J, Ongecha J, Ongas MO, Koskei N, Bull PC, Hodgson SH, Kivisi C, Imwong M, Murphy SC, Ogutu B, Tarning J, Winterberg M, Williams TN. 2023. Anti-merozoite antibodies induce natural killer cell effector function and are associated with immunity against malaria. Sci Transl Med. 15(682):eabn5993. https://doi.org/10.1126/scitranslmed.abn5993. PMID: 36753561.
  • Richie TL, Preston Church LW, Murshedkar T, Billingsley PF, James ER, Chen M, Abebe Y, Chakravarty S, KC N, Dolberg D, Healy SA, Diawara H, Sissoko MS, Sagara I, Cook DM, Epstein JE, Mordmüller B, Kapulu M, Kreidenweiss A, Franke-Fayard B, Agnandji ST, López Mikue MA, McCall MBB, Steinhardt L, Oneko M, Olotu A, Vaughan AM, Kublin JG, Murphy SC, Jongo S, Tanner M, Sirima SB, Laurens MB, Daubenberger C, Silva JC, Lyke KE, Janse CJ, Roestenberg M, Sauerwein RW, Abdulla S, Dicko A, Kappe SHI, Sim BKL, Duffy PE, Kremsner  PG, Hoffman SL. 2023. Sporozoite immunization: innovative translational science to support the fight against malaria. Expert Rev Vaccines. https://doi.org/10.1080/14760584.2023.2245890. PMID: 37571809.
  • Adissu W, Brito M, Garbin E, Macedo M, Monteiro W, Mukherjee S, Myburg J, Alam MS, Bancone G, Bansil P, Pal S, Sharma A, Zobrist S, Bryan A, Chu CS, Das S, Domingo GJ, Hann A, Kublin J, Lacerda MVG, Layton M, Ley B, Murphy SC, Nosten F, Pereira D, Price RN, Talukdar A, Yilma D, Gerth-Guyette E. 2023. Clinical performance validation of the STANDARD G6PD Test: A multi-country pooled analysis. PLOS Neglect Trop Dis 17(10): e0011652. https://doi.org/10.1371/journal.pntd.0011652.
  • Kayentao K, Ongoiba A, Preston AC, Healy SA, Hu Z, Skinner J, Doumbo S, Wang J, Cisse H, Doumtabe D, Traore A, Traore H, Djiguiba A, Li S, Peterson ME, Telscher S, Idris AH, Adams WC, McDermott AB, Narpala S, Lin BC, Serebryannyy L, Hickman SP, McDougal AJ, Vazquez S, Reiber M, Stein JA, Gall JG, Carlton K, Schwabl P, Traore S, Keita M, Zéguimé A, Ouattara A, Doucoure M, Dolo A, Murphy SC, Neafsey DE, Portugal S, Djimdé A, Traore B, Seder RA, Crompton PD, Mali Malaria mAb Trial Team. 2024. Subcutaneous Administration of a Monoclonal Antibody to Prevent Malaria in Children. N Engl J Med. 390(17):1549-59. PMID: 38669354.

Malaria Parasite Biology & Pathogenesis 
  • Murphy SC, Breman JG. 2001. Gaps in the childhood malaria burden in Africa: cerebral malaria, neurological sequelae, anemia, respiratory distress, hypoglycemia and complications of pregnancy. Am J Trop Med Hyg. 64(Supp 1-2):57-67. (https://doi.org/10.4269/ajtmh.2001.64.57)
  • Murphy SC, Samuel BU, Harrison T, Speicher KD, Speicher DW, Reid ME, Prohaska R, Low PS, Tanner MJ, Mohandas N, Haldar K. 2004. Erythrocyte detergent-resistant membrane proteins: their characterization and selective uptake during malarial infection. Blood. 103(5):1920-8. (https://doi.org/10.1182/blood-2003-09-3165)
  • Murphy SC, Hiller NL, Harrison T, Lomasney JW, Mohandas N, Haldar K. 2006. Lipid rafts and malaria parasite infection of erythrocytes (review). Molecular Membrane Biology. 23(1):81-88. (https://doi.org/10.1080/09687860500473440)
  • Murphy SC, Harrison T, Lomasney J, Hamm H, Mohandas N, Haldar K. 2006. Erythrocyte G protein as a novel target for malarial chemotherapy. PLoS Med. 3(12):e528. (https://doi.org/10.1371/journal.pmed.0030528)
  • Murphy SC, Fernandez-Pol S, Chung PH, Murthy SNP, Milne SB, Salomao M, Brown HA, Lomasney JW, Narla M, Haldar K. 2007. Cytoplasmic remodeling of erythrocyte raft lipids during infection by the human malaria parasite Plasmodium falciparum. Blood. 110(6):2132-9. (https://doi.org/10.1182/blood-2007-04-083873)
  • Marvin RG, Wolford JL, Kidd MJ, Murphy S, Ward J, Que EL, Mayer ML, Penner-Hahn JE, Haldar K, O'Halloran TV. 2012. Fluxes in "free" and total zinc are essential for progression of intraerythrocytic stages of Plasmodium falciparum. Chem Biol. 19(6):731-41. (https://doi.org/10.1016/j.chembiol.2012.04.013)

Studies of Other Pathogens (Theileria, Mycobacterium, etc.)
  • Fry LM, St all. 2019. Gene gun DNA immunization of cattle induces humoral and CD4 T-cell-mediated immune responses against the Theileria parva polymorphic immunodominant molecule. Vaccine 37(12):1546. (https://doi.org/10.1016/j.vaccine.2019.02.009)
  • Chang M, Venkatasubramanian S, Barrett H, Urdahl KB, Weigel KM, Cangelosi GA, Shah JA, Saha A, Feng L, Adams KN, Sherman DR, Smith N, Seshadri C, Kublin JG, Murphy SC. 2024. Molecular detection of pre-ribosomal RNAs of Mycobacterium bovis Bacille Calmette-Guérin and Mycobacterium tuberculosis to enhance pre-clinical tuberculosis drug and vaccine development. Diag Microbiol Infect Dis 108(1):116106. PMID: 37931386; https://doi.org/10.1016/j.diagmicrobio.2023.116106.
  • Church EC, Bishop E, Fiore-Gartland A, Yu KKQ, Chang M, Jones RM, Brache JK, Ballweber Fleming L, Phan JM, Makatsa MS, Heptinstall J, Chiong K, Dintwe O, Naidoo A, Voillet V, Mayer-Blackwell K, Nwanne G, Andersen-Nissen E, Vary JC Jr, Tomaras GD, McElrath MJ, Sherman DR, Murphy SC, Kublin JG, Seshadri C. 2024. Probing dermal immunity to mycobacteria through a controlled human infection model. Immunohorizons. 8(9):695-711. http://doi.org/10.4049/immunohorizons.2400053. PMID: 39283647

Ethics and Global Health Policy Papers
  • Murphy SC. 2006. Malaria and global infectious diseases: why should we care? Virtual Mentor. 8:245-250. (link)
  • Kraft SA, Duenas D, Shipman KJ, Kublin JG, Murphy SC, Shah SK. 2018. Exploring ethical concerns about high payments for human infection challenge studies: A qualitative study of participants’ motivations, perceptions of risk and payment, and potential deception in a controlled human malaria infection study J Empir Res Hum Res Ethics 1556264618820219 (https://doi.org/10.1177/1556264618820219) 
  • Shah SK, Miller FG, Darton TC, Duenas D, Emerson C, Fernandez Lynch H, Jamrozik E, Jecker NS, Kamuya D, Kapulu M, Kimmelman J, MacKay D, Memoli MJ, Murphy SC, Palacios R, Richie TL, Roestenberg M, Saxena M, Saylor K, Selgelid MJ, Vaswani V, Rid A. 2020. Responding to the COVID-19 pandemic: Ethical considerations for conducting controlled human infection studies. 2020. Science. eabc1076 (https://doi.org/10.1126/science.abc1076). (and our response to letter by Nir et al. is at https://doi.org/10.1126/science.abc9380. 
  • Murphy SC, Duenas DM, Richie TL, Shah SK. 2020. Reexamining the categorical exclusion of pediatric participants from controlled human infection trials. Bioethics. 2020 Jul 26. https://doi.org/10.1111/bioe.12788. PMID: 32715497.​


Picture
Sunset from Camp Orkila on Orcas Island, one of Washington's beautiful San Juan Islands.